Name: Anti-Integrin alpha V antibody [EPR16800]
Brand: RabMAb
SKU: ab179475
Rating: 5 (5 reviews)

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Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16800] to Integrin alpha V
Suitable for: WB, IP, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

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Primary

Protein

Secondary

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Primary - Rabbit Anti-Integrin alpha V antibody [EPR16800] (ab179475)
WB, IP, ICC/IF, IHC-P

Protein - Recombinant Human Integrin alpha V protein (Tagged) (ab114240)
ELISA, SDS-PAGE, WB

Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

View more associated products

Product name

Anti-Integrin alpha V antibody [EPR16800]
See all Integrin alpha V primary antibodies
Description

Rabbit monoclonal [EPR16800] to Integrin alpha V
Host species

Rabbit
Tested applications

Suitable for: WB, IP, ICC/IF, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human Integrin alpha V aa 1-250. The exact sequence is proprietary.
Database link: P06756
Positive control
- WB: HUVEC, HT-29, A549, C6 and NIH/3T3 whole cell lysates; Human fetal kidney and fetal brain lysates; Mouse brain, kidney and spleen lysates; Rat brain and kidney lysates. IHC: Human kidney, human transitional cell carcinoma of bladder and mouse kidney tissues. ICC/IF: A549 cells. IP: A549 whole cell extract.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16800
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab179475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/5000. Detects a band of approximately 125, 135 kDa (predicted molecular weight: 116 kDa).
IP		1/40.
ICC/IF		1/500.
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similarities

Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Cellular localization

Membrane.
Target information above from: UniProt accession P06756 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3685 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 296456 Rat
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P43406 Mouse
- Unigene: 436873 Human
- Unigene: 227 Mouse
Alternative names
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
- DKFZp686A08142 antibody
- Integrin alpha five antibody
- integrin alpha V beta 3 antibody
- Integrin alpha-5 antibody
- integrin alpha-V antibody
- Integrin alpha-V light chain antibody
- integrin alphaVbeta3 antibody
- integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
- ITAV_HUMAN antibody
- ITGAV antibody
- MSK 8 antibody
- Msk8 antibody
- Vitronectin receptor subunit alpha antibody
- VNRA antibody
- VTNR antibody
see all

Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/50000 dilution

Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysates
Lane 2 : HT-29 (Human colorectal adenocarcinoma cells) whole cell lysates
Lane 3 : A549 (Human lung carcinoma) whole cell lysates

Lysates/proteins at 20 μg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa
Observed band size: 125 kDa
why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

Membrane and weak cytoplasm staining on human transitional cell carcinoma of bladder is observed. Counterstained with hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Integrin alpha V was immunoprecipitated from 1 mg of A549 (Human lung carcinoma) whole cell extract with ab179475 at 1/40 dilution. Western blot analysis was performed from the immunoprecipitate using ab179475 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1: A549 whole cell extract.

Lane 2: PBS instead of A549 whole cell extract.

Blocking/Dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling Integrin alpha V with ab179475 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor^® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green).

Confocal image showing membrane and cytoplasm staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor^®594 secondary antibody) at 1/500 dilution (red).

The negative controls are as follows:

-ve control 1: ab179475 at 1/500 dilution followed by ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution.

-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor^®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

Lane 1 : Human fetal kidney lysates
Lane 2 : Human fetal brain lysates

Lysates/proteins at 10 μg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 116 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

Membrane and cytoplasm staining on human kidney tubules is observed. Counterstained with hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Mouse kidney lysates
Lane 3 : Mouse spleen lysates
Lane 4 : Rat brain lysates
Lane 5 : Rat kidney lysates
Lane 6 : C6 (Rat glial tumor cells) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lane 8 : Raji (Human Burkitt's lymphoma cell line) whole cell lysates

Lysates/proteins at 10 μg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa
Observed band size: 125,135 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary at 1/500 dilution.

Membrane and cytoplasm staining on mouse kidney tubule and glomerulus is observed. Counterstained with hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)This image is courtesy of an anonymous Abreview.

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/2000 dilution (8 hours at 4°C)

All lanes : Mouse skin whole cell lysate

Lysates/proteins at 40 μg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG(H+L)-HRP at 1/5000 dilution

Developed using the ECL technique.

Predicted band size: 116 kDa

Exposure time: 25 seconds

Diluent: 2% BSA in 1X TBST.

Blocked using 5% BSA for 1 hour at RT.

See Abreview
Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Publishing research using ab179475? Please let us know so that we can cite the reference in this datasheet.

ab179475 has been referenced in 32 publications.

Bi J et al. Irisin reverses intestinal epithelial barrier dysfunction during intestinal injury via binding to the integrin aV?5 receptor. J Cell Mol Med 24:996-1009 (2020). PubMed: 31701659
Nie Z et al. Multi-mode biodegradable tumour-microenvironment sensitive nanoparticles for targeted breast cancer imaging. Nanoscale Res Lett 15:81 (2020). PubMed: 32297036
Kim BH et al. STAT3 Inhibitor ODZ10117 Suppresses Glioblastoma Malignancy and Prolongs Survival in a Glioblastoma Xenograft Model. Cells 9:N/A (2020). PubMed: 32183406
Chen F et al. Molecular phenotyping and image-guided surgical treatment of melanoma using spectrally distinct ultrasmall core-shell silica nanoparticles. Sci Adv 5:eaax5208 (2019). PubMed: 31840066
Tuguzbaeva G et al. PEP06 polypeptide 30 is a novel cluster-dissociating agent inhibiting a v integrin/FAK/Src signaling in oral squamous cell carcinoma cells. Acta Pharm Sin B 9:1163-1173 (2019). PubMed: 31867162

View all Publications for this product

Customer reviews and Q&As

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Application

Immunocytochemistry/ Immunofluorescence

Sample

Rat Cell (Rat hepatocytes)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Rat hepatocytes

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Sep 10 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (22Rv1 - prostate cancer cells)

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Loading amount

25 µg

Specification

22Rv1 - prostate cancer cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Aug 31 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (PC-3 - prostate cancer cells)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

PC-3 - prostate cancer cells

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Aug 28 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Human skin SCC)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate buffer

Permeabilization

Specification

Human skin SCC

Blocking step

Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Fixative

Paraformaldehyde

Mr. Yong Sun Lee

Verified customer

Submitted Jun 27 2018

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse skin)

Gel Running Conditions

Reduced Denaturing (8%)

Loading amount

40 µg

Specification

Mouse skin

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 20 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Certificate of Compliance

References (32)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha V antibody [EPR16800]

Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V antibody [EPR16800]

Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]