Recombinant Anti-Beclin 1 antibody [EPR19662] (ab207612)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19662] to Beclin 1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Beclin 1 antibody [EPR19662]
See all Beclin 1 primary antibodies -
Description
Rabbit monoclonal [EPR19662] to Beclin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Beclin 1 aa 1-200. The exact sequence is proprietary.
Database link: Q14457 -
Positive control
- WB: Human fetal brain, fetal heart and fetal kidney lysates; HeLa, HepG2, 293, MCF7, Jurkat, Raji, C6, RAW264.7, PC-12, NIH/3T3, A20 and C2C12 whole cell lysates; rat brain, kidney and spleen lysates; mouse brain, heart and spleen lysates. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19662 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab207612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). | |
| IP | 1/30. |
Target
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Function
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the beclin family. -
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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Database links
- Entrez Gene: 8678 Human
- Entrez Gene: 56208 Mouse
- Entrez Gene: 114558 Rat
- Omim: 604378 Human
- SwissProt: Q14457 Human
- SwissProt: O88597 Mouse
- SwissProt: Q91XJ1 Rat
- Unigene: 716464 Human
see all -
Alternative names
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
Images
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![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](http://www.45shebe.icu/ps/products/207/ab207612/Images/ab207612-261167-anti-beclin-1-antibody-epr19662-western-blot.jpg)
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 μg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](http://www.45shebe.icu/ps/products/207/ab207612/Images/ab207612-261165-anti-beclin-1-antibody-epr19662-western-blot.jpg)
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 10 μg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 30 seconds; Lane 5: 15 seconds; Lane 6: 15 seconds.
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![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](http://www.45shebe.icu/ps/products/207/ab207612/Images/ab207612-261164-anti-beclin-1-antibody-epr19662-western-blot.jpg)
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)Lanes 1-4 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
Lanes 5-6 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 5 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate
Lane 6 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lysates/proteins at 10 μg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 5 seconds; Lane 5: 10 seconds; Lane 6: 30 seconds.
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![Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)](http://www.45shebe.icu/ps/products/207/ab207612/Images/ab207612-261163-anti-beclin-1-antibody-epr19662-western-blot.jpg)
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat kidney lysate
Lane 3 : Rat spleen lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse heart lysate
Lane 6 : Mouse spleen lysate
Lysates/proteins at 10 μg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/6: 5 seconds; Lane 2/3: 15 seconds; Lane 4: 3 seconds; Lane 5: 10 seconds.
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![Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)](http://www.45shebe.icu/ps/products/207/ab207612/Images/ab207612-261162-anti-beclin-1-antibody-epr19662-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)Beclin 1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab207612 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab207612 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10µg (Input).
Lane 2: ab207612 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207612 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-Beclin 1 antibody [EPR19662] (ab207612)](http://www.45shebe.icu/ps/products/207/ab207612/Images/ab207612-9-benefits-of-recombinant-antibodies.png)
Anti-Beclin 1 antibody [EPR19662] (ab207612)
Protocols
References (67)
ab207612 has been referenced in 67 publications.
- Qi H et al. MSTN Attenuates Cardiac Hypertrophy through Inhibition of Excessive Cardiac Autophagy by Blocking AMPK /mTOR and miR-128/PPAR?/NF-?B. Mol Ther Nucleic Acids 19:507-522 (2020). PubMed: 31923740
- Xia H et al. ube3d, a New Gene Associated with Age-Related Macular Degeneration, Induces Functional Changes in Both In?Vivo and In?Vitro Studies. Mol Ther Nucleic Acids 20:217-230 (2020). PubMed: 32200270
- Yang Z et al. Upregulated NTF4 in colorectal cancer promotes tumor development via regulating autophagy. Int J Oncol 56:1442-1454 (2020). PubMed: 32236587
- Chen X et al. miR-155 facilitates calcium oxalate crystal-induced HK-2 cell injury via targeting PI3K associated autophagy. Exp Mol Pathol 115:104450 (2020). PubMed: 32417393
- Qin H & Cai J Effect of Periostin Silencing on the Autophagy of Osteoblasts. Cell Reprogram 21:122-128 (2019). PubMed: 31145651
